Enhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus
نویسندگان
چکیده
منابع مشابه
pcd mutants of Streptomyces clavuligerus still produce cephamycin C.
Biosynthesis of cephamycin C in Streptomyces clavuligerus involves the initial conversion of lysine to alpha-aminoadipic acid. Lysine-6-aminotransferase and piperideine-6-carboxylate dehydrogenase carry out this two-step reaction, and genes encoding each of these enzymes are found within the cephamycin C gene cluster. However, while mutation of the lat gene causes complete loss of cephamycin pr...
متن کاملCoordinate production of cephamycin c and clavulanic acid by Streptomyces clavuligerus.
Production of cephamycin c and clavulanic acid by Streptomyces clavuligerus was investigated using different media in shake flask condition. Highest cell growth (3.8 g/L) was observed in glycerol, sucrose, proline and glutamic acid (GSPG) medium. Although, GSPG medium supported maximum growth, it was least effective for the synthesis of both cephamycin and clavulanic acid. Yield of cephamycin a...
متن کاملPRODUCTION OF CLAVULANIC ACID AND CEPHAMYCIN C BY Streptomyces clavuligerus UNDER DIFFERENT FED-BATCH CONDITIONS
The effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L. Highest CephC production (566.5 mg.L) was obtained in fed-batch cultivation with glycerol feeding. In fed-batc...
متن کاملStudies on the Biosynthetic Pathways of Clavulanic Acid and Cephamycin C in Streptomyces clavuligerus
Mackenzie, A., Studies on the Biosynthetic Pathways of Clavulanic Acid and Cephamycin C in Streptomyces clavuligerus. Doctoral dissertation. ISSN 1652-6880 ISBN 978-91-576-7318-3 The discovery of penicillin, a β-lactam antibiotic, changed the way humans thought about infectious disease. Unfortunately, the widespread use of antibiotics has lead to a concomitant increase in bacterial resistance t...
متن کاملDissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus.
Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indica...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2013
ISSN: 1471-2180
DOI: 10.1186/1471-2180-13-296